Clotrimazole (CLZ) and dexamethasone (DEX) is available as cream formulation and no any method have been reported yet. So aim of present research work is to develop a simple, sensitive and reproducible method for simultaneous estimation of both drugs by first order derivative spectrophotometric method. Methanol was used as a solvent for analysis. The method used has been tested for accuracy and precision for the estimation of both the drugs. In this method 239.00 nm, the zero crossing point of DEX was selected for determination of CLZ and 252.80 nm, the zero crossing point of CLZ was selected for determination of DEX. The regression analysis data for the calibration plot showed good linearity in the range 100-500 µg/ml (R2 = 0.9985) for CLZ and 4-20 µg/ml (R2 = 0.9987) for DEX. The LOD for CLZ and DXM were 8.66 µg/ml and 0.49 µg/ml and LOQ were found to be 26.28 µg/ml and 1.45 µg/ml respectively. These methods were applied to the assay of the drugs in marketed formulation, which were found in the range of 98% to 100% for both CLZ and DEX. Statistical analysis proves that the method is reproducible and selective for the simultaneous estimation of CLZ and DEX. The results were found to be within acceptance criteria according to ICH Q2 R1 guidelines.
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